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Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Through our innovative reagents, we empower researchers to make groundbreaking discoveries and achieve breakthroughs in genetic research.

Empowering Breakthroughs in Genetic Research

​Artificial Chaperone-assisted Hybridization Reagent ​

Anac: Accelerating Life Science Research

Anac reagents are Artificial Nucleic Acid Chaperones for accurate hybridization and folding of nucleic acids. They can accelerate gene-related reactions by hundreds to tens of thousands of times, significantly reducing research time and costs.

Anac to Boost DNA Technology

Nanodevices

・Nanoconstructs

・Nanomachines

​・Molecular computing

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

DNA analysis

・Geno-typing

・DNA array,DNA chip

​・mRNA,miRNA sensing

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Nucleic acids medicines

・Antisense

・Antigene

​・siRNA

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computerc1-90fc-40d1-9d49-586493a35c61.png

Functional nucleic acids

・Aptamer

・(Deoxy)Ribozyme

​・DNA template reaction

Promotion of duplex, triplex and quadruplex formation

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

DTm ~ 20 ˚C

dsDNA

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

complex

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
image.png
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

ssDNAs

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Acceleration

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

dsDNA, triplex…

Strand exch. activation

dsDNA

image.png
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

quadruplex

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Structural transition

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Right handed

DNA

​（B-type）

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Left handed

DNA

​（Z-type）

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

and

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Polymorphic

folding

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Monomorphic

folding

Intra vs. Inter molecular  structural conversion

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Intramolecular

stem-loop

PA

Intermolecular
dsDNA

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Intramolecular
quadruplex

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

dsDNA

Polym. J., 2019

https://www.nature.com/articles/s41428-019-0210-7

Biomaterials, 2020

https://www.sciencedirect.com/science/article/abs/pii/S0142961220303963?via%3Dihub

Boost DNA Computing with Anac

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

AND gate

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

0

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

0.0

Adv. Funct. Mater., 2018

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201707406

OR gate

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

0

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

0.0

The copolymer promoted molecular operation without loss of acuracy.

The copolymer acts as “Molecular Booster” for DNA computing

Adv. Funct. Mater., 2018

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201707406

Boost DNA Enzyme with Anac

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Without Anac

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

With Anac

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

With Anac

Without Anac

Biomaterials, 2019

https://www.sciencedirect.com/science/article/abs/pii/S0142961219306349

Biosens. Bioelecton., 2020

https://www.sciencedirect.com/science/article/pii/S0956566320303778?via%3Dihub

Boost Peroxidase-DNAzyme with Anac

Boost Peroxidase-DNAzyme with Anac.png

Biomacromolecules 2018

https://pubs.acs.org/doi/10.1021/acs.biomac.8b00201

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Anac(-)
Anac(+)

Biomater. Sci., 2021,9, 6142

https://pubs.rsc.org/en/content/articlelanding/2021/bm/d1bm00949d

Protect DNA Computing

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Without Anac
With Anac

Fig.  DNA logic gate operation in the presence of nuclease (DNase I : 26.5 Kunitz unit)

Chaperone polymer protected DNA components from DNase　　　

Adv. Funct. Mater., 2018

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201707406

Boost DNA Circuit (Hybridization Chain Reaction)

miRNA (let-7a) as initiator

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Anac

100 nM let-7a, 500 nM H1-l7, 500 nM H2-l7, Anac (N/P=0 or 10), 25°C for 1 h.

図62.png

Anac(+)

Anac(-)

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

20 nM let-7a, 100 nM H1-l7, 100 nM FQ-H2-l7, Anac (N/P=0 or 10), 25°C.

Anac accelerates different HCR circuits initiated by either RNA or DNA initiator

ACS Appl. Mater. Interfaces, 2022

https://pubs.acs.org/doi/10.1021/acsami.2c11548

Small, 2023

https://onlinelibrary.wiley.com/doi/10.1002/smll.202304091

Boost Genotyping with Anac

Simple & quick genotyping
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Nature Mater. 2003

https://doi.org/10.1002/smll.202301219

Boost DNA Nanomachines with Anac

DNA nanomachine boosting
Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Fuel 1 / Fuel 2

Fuel 2

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

Without Anac

Hybridization enhancer DNA nanomachine DNA circuit DNAzyme Ribozyme Nucleic acid chaperone Cationic polymer Strand replacement Strand exchange Hybridization promoter DNA nanodevice DNA robot DNA logic gate DNA computer

With Anac

Nano Lett. 2007
https://pubs.acs.org/doi/abs/10.1021/nl0626232

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